There are 43 former Division I players in the 2016 MLB League Championship Series. There are 34 schools represented. Georgia and Long Beach State lead with three players. There are two players from Arizona State, Auburn, Dallas Baptist, Texas A&M and UCLA.
Toronto leads all teams with 13 former Division I players on its rosters. Cleveland and Los Angeles both have 11, while Chicago has eight.
| Arizona State | Andre | Ethier | OF | Dodgers |
| Arizona State | Jason | Kipnis | 2B | Indians |
| Auburn | Grant | Dayton | LHP | Dodgers |
| Auburn | Josh | Donaldson | 3B | Blue Jays |
| Cal State Fullerton | Justin | Turner | 3B | Dodgers |
| Dallas Baptist | Ryan | Goins | 2B | Blue Jays |
| Dallas Baptist | Ben | Zobrist | 2B | Cubs |
| Dartmouth | Kyle | Hendricks | RHP | Cubs |
| Duke | Marcus | Stroman | RHP | Blue Jays |
| Florida | David | Ross | C | Cubs |
| Georgia | Josh | Fields | RHP | Dodgers |
| Georgia | Justin | Grimm | RHP | Cubs |
| Georgia | Alex | Wood | LHP | Dodgers |
| High Point | Cody | Allen | RHP | Indians |
| Kentucky | Joe | Blanton | RHP | Dodgers |
| Long Beach State | Marco | Estrada | RHP | Blue Jays |
| Long Beach State | Bryan | Shaw | RHP | Indians |
| Long Beach State | Troy | Tulowitzki | SS | Blue Jays |
| Maryland | Brett | Cecil | LHP | Blue Jays |
| Miami (Fla.) | Yasmani | Grandal | C | Dodgers |
| Michigan | Rich | Hill | LHP | Dodgers |
| Missouri | Rob | Zastryzny | LHP | Cubs |
| North Carolina | Andrew | Miller | LHP | Indians |
| Northwestern | J.A. | Happ | LHP | Blue Jays |
| Notre Dame | Jeff | Manship | RHP | Indians |
| Ole Miss | Chris | Coghlan | OF | Cubs |
| Oregon State | Darwin | Barney | 2B | Blue Jays |
| Sam Houston State | Ryan | Tepera | RHP | Blue Jays |
| San Diego | Kris | Bryant | 3B | Cubs |
| Seton Hall | Jason | Grilli | RHP | Blue Jays |
| South Carolina | Justin | Smoak | 1B | Blue Jays |
| South Florida | Dan | Otero | RHP | Indians |
| Stetson | Corey | Kluber | RHP | Indians |
| TCU | Jake | Arrieta | RHP | Cubs |
| Tennessee | Andrew | Toles | OF | Dodgers |
| Texas A&M | Tyler | Naquin | OF | Indians |
| Texas A&M | Ross | Stripling | RHP | Dodgers |
| Texas Tech | Josh | Tomlin | RHP | Indians |
| Tulane | Aaron | Loup | LHP | Blue Jays |
| UC Davis | Joe | Biagini | RHP | Blue Jays |
| UCLA | Trevor | Bauer | RHP | Indians |
| UCLA | Chase | Utley | 2B | Dodgers |
| Virginia | Brandon | Guyer | OF | Indians |