Nearly two-thirds of the players taken on Day 1 of the 2019 MLB Draft were NCAA Division I college players.
There were 51 college players taken out of 78 selections through the first two rounds of the draft. Seven of the top 10 picks and 21 of the top 32 were college players.
There were players selected from 38 different schools. There were 12 teams with multiple selections, led by UCLA with three.
| Rd. | Pick | Team | Player | School | Pos. |
| 1 | 1 | Baltimore | Adley Rutschan | Oregon State | C |
| 1 | 3 | Chicago White Sox | Andrew Vaughn | California | 1B |
| 1 | 4 | Miami | JJ Bleday | Vanderbilt | OF |
| 1 | 7 | Cincinnati | Nick Lodolo | TCU | P |
| 1 | 8 | Texas | Josh Jung | Texas Tech | 3B |
| 1 | 9 | Atlanta | Shea Langeliers | Baylor | C |
| 1 | 10 | San Francisco | Hunter Bishop | Arizona State | OF |
| 1 | 11 | Toronto | Alek Manoah | West Virginia | P |
| 1 | 14 | Philadelphia | Bryson Stott | UNLV | SS |
| 1 | 15 | Anaheim | Will Wilson | NC State | SS |
| 1 | 19 | St. Louis | Zack Thompson | Kentucky | P |
| 1 | 20 | Seattle | George Kirby | Elon | P |
| 1 | 21 | Atlanta | Braden Shewmake | Texas A&M | SS |
| 1 | 22 | Tampa Bay | Greg Jones | UNC Wilmington | SS |
| 1 | 23 | Colorado | Michael Toglia | UCLA | 1B |
| 1 | 25 | LA Dodgers | Kody Hoese | Tulane | 3B |
| 1 | 27 | Chicago Cubs | Ryan Jensen | Fresno State | P |
| 1 | 28 | Milwaukee | Ethan Small | Mississippi State | P |
| 1 | 29 | Oakland | Logan Davidson | Clemson | SS |
| 1 | 31 | LA Dodgers | Michael Busch | North Carolina | 2B |
| 1 | 32 | Houston | Korey Lee | California | C |
| 1C | 34 | Arizona | Drey Jameson | Ball State | P |
| CBA | 35 | Miami | Kameron Misner | Missouri | CF |
| CBA | 38 | New York Yankees | TJ Sikkema | Missouri | P |
| CBA | 39 | Minnesota | Matt Wallner | Southern Miss | RF |
| CBA | 40 | Tampa Bay | Seth Johnson | Campbell | P |
| CBA | 41 | Texas | Davis Wendzel | Baylor | 3B |
| 2 | 43 | Arizona | Cameron Cannon | Arizona | SS |
| 2 | 44 | Kansas City | Brady McConnell | Florida | SS |
| 2 | 47 | Detroit | Nick Quintana | Arizona | 3B |
| 2 | 50 | Texas | Ryan Garcia | UCLA | P |
| 2 | 51 | San Francisco | Logan Wyatt | Louisville | 1B |
| 2 | 54 | Minnesota | Matt Canterino | Rice | P |
| 2 | 56 | Arizona | Ryne Nelson | Oregon | P |
| 2 | 57 | Pittsburgh | Matt Gorski | Indiana | CF |
| 2 | 59 | Seattle | Brandon Williamson | TCU | P |
| 2 | 60 | Atlanta | Beau Philip | Oregon State | SS |
| 2 | 61 | Tampa Bay | John Doxakis | Texas A&M | P |
| 2 | 62 | Colorado | Aaron Schunk | Georgia | 3B |
| 2 | 64 | Chicago Cubs | Chase Strumpf | UCLA | 2B |
| 2 | 66 | Oakland | Tyler Baum | North Carolina | P |
| 2 | 67 | New York Yankees | Josh Smith | LSU | 2B |
| 2 | 68 | Houston | Grae Kessinger | Ole Miss | SS |
| CBB | 70 | Kansas City | Alec Marsh | Arizona State | P |
| CBB | 71 | Baltimore | Kyle Stowers | Stanford | CF |
| CBB | 72 | Pittsburgh | Jared Triolo | Houston | 3B |
| CBB | 73 | San Diego | Logan Driscoll | George Mason | C |
| CBB | 74 | Arizona | Tommy Henry | Michigan | P |
| CBB | 75 | Arizona | Dominic Fletcher | Arkansas | OF |
| CBB | 76 | Seattle | Isaiah Campbell | Arkansas | P |
| CBB | 77 | Colorado | Karl Kauffmann | Michigan | P |