There are 87 former Division I players in the 2016 MLB Divisional Series. There are 61 schools represented and Arizona State leads with five players. There are four players from Long Beach State and UCLA, three from Florida State, South Carolina and Virginia, as well as two from Auburn, Georgia, Northwestern, Notre Dame, Ole Miss, Rice, San Diego, Texas A&M, Texas Tech and UC Riverside.
Toronto and Washington both have 14 former Division I players on their rosters. Cleveland and San Francisco both have 12, Boston and Los Angeles both had 11, Chicago has eight and Texas has five.
| Arizona | Mark | Melancon | RHP | Nationals |
| Arizona State | Austin | Barnes | C | Dodgers |
| Arizona State | Tony | Barnette | RHP | Rangers |
| Arizona State | Andre | Ethier | OF | Dodgers |
| Arizona State | Jason | Kipnis | 2B | Indians |
| Arizona State | Dustin | Pedroia | 2B | Red Sox |
| Arkansas | Andrew | Benintendi | OF | Red Sox |
| Auburn | Grant | Dayton | LHP | Dodgers |
| Auburn | Josh | Donaldson | 3B | Blue Jays |
| Austin Peay | Shawn | Kelley | RHP | Nationals |
| Cal State Fullerton | Justin | Turner | 3B | Dodgers |
| Coastal Carolina | Tommy | La Stella | 2B | Cubs |
| Connecticut | Matt | Barnes | RHP | Red Sox |
| Creighton | Ty | Blach | LHP | Giants |
| Dallas Baptist | Ben | Zobrist | 2B | Cubs |
| Dartmouth | Kyle | Hendricks | RHP | Cubs |
| Duke | Marcus | Stroman | RHP | Blue Jays |
| Florida | David | Ross | C | Cubs |
| Florida State | Stephen | Drew | 2B | Nationals |
| Florida State | Buster | Posey | C | Giants |
| Florida State | Devon | Travis | 2B | Blue Jays |
| Georgia | Josh | Fields | RHP | Dodgers |
| Georgia | Justin | Grimm | RHP | Cubs |
| High Point | Cody | Allen | RHP | Indians |
| Illinois | Tanner | Roark | RHP | Nationals |
| Jacksonville | Daniel | Murphy | 2B | Nationals |
| Kent State | Travis | Shaw | 3B | Red Sox |
| Kentucky | Joe | Blanton | RHP | Dodgers |
| Long Beach State | Danny | Espinosa | SS | Nationals |
| Long Beach State | Marco | Estrada | RHP | Blue Jays |
| Long Beach State | Bryan | Shaw | RHP | Indians |
| Long Beach State | Troy | Tulowitzki | SS | Blue Jays |
| LSU | Aaron | Hill | 3B | Red Sox |
| Maryland | Brett | Cecil | LHP | Blue Jays |
| Miami (Fla.) | Yasmani | Grandal | C | Dodgers |
| Michigan | Rich | Hill | LHP | Dodgers |
| Mississippi State | Mitch | Moreland | 1B | Rangers |
| Missouri | Max | Scherzer | RHP | Nationals |
| Missouri State | Brad | Ziegler | RHP | Red Sox |
| NC State | Trea | Turner | OF | Nationals |
| Nevada | Chris | Gimenez | C | Indians |
| North Carolina | Andrew | Miller | LHP | Indians |
| Northwestern | J.A. | Happ | LHP | Blue Jays |
| Northwestern | George | Kontos | RHP | Giants |
| Notre Dame | Jeff | Manship | RHP | Indians |
| Notre Dame | Jeff | Samardzija | RHP | Giants |
| Ole Miss | Chris | Coghlan | OF | Cubs |
| Ole Miss | Drew | Pomeranz | LHP | Red Sox |
| Oregon State | Darwin | Barney | 2B | Blue Jays |
| Princeton | Danny | Barnes | RHP | Blue Jays |
| Rice | Brock | Holt | OF | Red Sox |
| Rice | Anthony | Rendon | 3B | Nationals |
| Sam Houston State | Ryan | Tepera | RHP | Blue Jays |
| San Diego | Kris | Bryant | 3B | Cubs |
| San Diego | Sammy | Solis | LHP | Nationals |
| Seton Hall | Jason | Grilli | RHP | Blue Jays |
| South Carolina | Jackie | Bradley Jr. | OF | Red Sox |
| South Carolina | Sam | Dyson | RHP | Rangers |
| South Carolina | Justin | Smoak | 1B | Blue Jays |
| South Dakota State | Blake | Treinen | RHP | Nationals |
| South Florida | Dan | Otero | RHP | Indians |
| St. John’s | Joe | Panik | 2B | Giants |
| Stetson | Corey | Kluber | RHP | Indians |
| TCU | Jake | Arrieta | RHP | Cubs |
| Tennessee | Andrew | Toles | OF | Dodgers |
| Texas | Brandon | Belt | 1B | Giants |
| Texas A&M | Tyler | Naquin | OF | Indians |
| Texas A&M | Ross | Stripling | RHP | Dodgers |
| Texas Tech | Josh | Tomlin | RHP | Indians |
| Texas Tech | Kelby | Tomlinson | 2B | Giants |
| Toledo | Jared | Hoying | OF | Rangers |
| Troy | Clint | Robinson | 1B | Nationals |
| Tulane | Aaron | Loup | LHP | Blue Jays |
| UC Davis | Joe | Biagini | RHP | Blue Jays |
| UC Riverside | Joe | Kelly | RHP | Red Sox |
| UC Riverside | Marc | Rzepczynski | LHP | Nationals |
| UCLA | Trevor | Bauer | RHP | Indians |
| UCLA | Trevor | Brown | C | Giants |
| UCLA | Brandon | Crawford | SS | Giants |
| UCLA | Chase | Utley | 2B | Dodgers |
| UL Lafayette | Jonathan | Lucroy | C | Rangers |
| UT Arlington | Hunter | Pence | OF | Giants |
| Vanderbilt | David | Price | LHP | Red Sox |
| Virginia | Brandon | Guyer | OF | Indians |
| Virginia | Javier | Lopez | LHP | Giants |
| Virginia | Ryan | Zimmerman | 1B | Nationals |
| Wichita State | Conor | Gillaspie | 3B | Giants |